The highly stereoselective bioreduction of ethyl cyclohexanone-2-carboxylate (1) on a kilogram scale is described.Ethyl(1S,2S)-trans-2-hydroxycyclohexane carboxylate (2) was prepared in 56% yield with a diastereomeric ratio (dr) of 99:1 and an enantiomeric ratio (er) of Accessories 99.5:0.5 after rectification.The cell growth and the biotransformation were performed in a large wave bioreactor equipped with a 200 l cell bag at Novartis Pharma AG, while the different purification steps were performed at the FHBB (Muttenz).
The fourth semester chemistry students at the FHBB were actively involved FRESH HAIR COLORING CREAM in the preparation and optimization of the entire process.